Tsuna has been hit by the Rebuke Bullet, which, according to Reborn, brings out the calm fighting will in him. Mukuro Rokudo ignores this and attacks Tsuna with Ken Joshima from behind. However, Tsuna easily grabs his head and defeats him. Mukuro uses Chikusa Kakimoto to attack with his needles but Tsuna knew that it was an illusion and attacks the true body, much to Mukuro's surprise. Reborn explains that the dormant Blood of Vongola within Tsuna has awakened allowing his internal limiters to be lifted and also to possess the Vongola Hyper Intuition. Reborn also adds that the gloves have another use.
Mukuro is confident that Tsuna will not harm Gokudera and Bianchi's bodies and attacks him with them. Tsuna defends himself from Gokudera and Bianchi's attacks and strikes their nerves to paralyze them. With his vessels defeated, Mukuro returns to his own body and uses his ugliest and most dangerous skill, the The Realm of Humans, which increases his battle aura, thereby strengthening him. Mukuro charges at Tsuna and overpowers him, sending him crashing to the wall. However, Tsuna activates the power of the gloves and readies himself to counterattack.
Note: Bold + Italicized = First Appearance